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Image Search Results
Journal: Regenerative Therapy
Article Title: Generation of a large-scale vascular bed for the in vitro creation of three-dimensional cardiac tissue
doi: 10.1016/j.reth.2019.10.001
Figure Lengend Snippet: Cardiac cell sheet implantation . (a) An endothelial cell network (evaluated from the expression of CD31, an endothelial cell marker) was clearly detected following the co-culture of green fluorescent protein (GFP)-expressing human umbilical vein endothelial cells (HUVECs) with adult normal human dermal fibroblasts (NHDFs) at a ratio of 10:1. cTnT: cardiac troponin T. (b) An endothelial cell network was not detected after the co-culture of cardiomyocytes derived from human induced pluripotent stem cells (hiPSCs) and GFP-positive HUVECs. CM: cardiomyocytes. (c) An endothelial cell network was created when cardiomyocytes were co-cultured with GFP-positive HUVECs and NHDFs at a ratio of 5:1:5. (d) Cardiomyocytes derived from hiPSCs were co-cultured with GFP-positive HUVECs in a temperature-responsive dish and harvested as a monolayer sheet. The harvested sheet shrank to approximately one-third of the dish diameter. (e) Three harvested cardiac cell sheets were layered, and this triple-layered cell sheet (broken circle) was placed onto a vascular bed created from porcine small intestine (day 0). The fabricated tissue construct was then subjected to perfusion culture for 6 days. (f) Needle electrodes were used to record surface action potentials from the implanted cardiac cell sheet (broken circle) after 6 days of perfusion culture. (g) Representative trace showing surface action potentials recorded from the implanted cardiac cell sheet after 6 days of perfusion culture. The implanted cardiac cell sheet was observed to beat spontaneously with a regular rhythm.
Article Snippet: The bioreactor was opened, and two
Techniques: Expressing, Marker, Co-Culture Assay, Derivative Assay, Cell Culture, Construct